Editing

The particles found can be further selected depending on their size and other properties.

Remember that if a particle is connected in 2 dimensions, then it can only exist within a slice. However, a particle connected in 3 dimensions can be connected across multiple slices. Particles can be edited using the following criteria by clicking the appropriate check boxes:

• Remove particles that contact the edge of the field of view. In 2 dimensions, if a particle touches the edge of the slice field-of-view, it will be removed. In 3 dimensions, if the particle touches the edge of the field of view in any slice, it will be removed. The top and bottom slices do not count as edges of the field of view in 3 dimensions (see the Note about connections in 3 dimensions.
• Max/min particle size. Particles that are bigger than or smaller than the given size can be retained, and all other particles rejected. If either of these mutually-exclusive options is selected, then be sure to set the maximum/minimum number of pixels in the # pixels in a particle field to the right.
• Biggest/smallest particles. Select a fixed number of particles that are either the biggest or smallest in the image. If either of these mutually-exclusive options is selected, then be sure to set the number of particles to select in the # particles field to the right.

Having decided on editing procedures to be applied, now preview or apply particle analysis.